757 CHROMOSOME MAPPING OF HUMAN GENES BY GENE PURIFICATION SOMATIC CELL HYBRIDIZATION AND RESTRICTION ENZYME ANLAYSIS
نویسندگان
چکیده
منابع مشابه
Assignment of LHX1 to human chromosome bands 17q11.2-->q12 by use of radiation hybrid mapping and somatic cell hybridization.
The human LHX1 gene encodes a protein that is a member of the LIM family of homeodomain proteins. LHX1 was originally cloned as an apparent homologue of the mouse Lhx1 gene and mapped to human chromosome 11p13→p12 (Dong et al., 1997). In the mouse, the Lhx1 gene is expressed in the developing kidney, and portions of the CNS where it is believed to be an essential regulator of the vertebrate hea...
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A series of mouse-human hybrids was prepared from mouse cells deficient in adenine phosphoribosyltransferase (EC 2.4.2.7) and normal human cells. The hybrids were made in medium containing adenine and alanosine, an antimetabolite known to inhibit de novo adenylic acid biosynthesis. The mouse cells, unable to utilize exogenous adenine, were killed in this medium, but the hybrids proliferated as ...
متن کاملAssignment of the gene for methylthioadenosine phosphorylase to human chromosome 9 by mouse-human somatic cell hybridization.
The purine and polyamine metabolic enzyme methylthioadenosine (MeSAdo) phosphorylase is abundant in normal cells and tissues but is lacking from many human and murine malignant cell lines and from cells of some human leukemias in vivo. To explore the genetic control of MeSAdo phosphorylase expression, we measured levels of the enzyme in somatic cell hybrids prepared by fusing MeSAdo phosphoryla...
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Glycerol-3-phosphate acyltransferase catalyzes the first step of glycerolipid biosynthesis. It plays a key role in the regulation of cellular triacylglycerol and phospholipid levels. There are two isoforms of glycerol-3-phosphate acyltransferase in mammals, a mitochondrial and a cytosolic form. The mitochondrial form (GPAM) prefers saturated fatty acyl-CoA as a substrate, whereas the cytosolic ...
متن کاملGene transfer by means of cell fusion. II. The mapping of 8 loci on human chromosome 1 by statistical analysis of gene assortment in somatic cell hybrids.
A method is described which should permit determination of the order and spacing of genes on all human chromosomes by the analysis of just one set of man-mouse hybrid cells. This method is used to determine the map of 8 loci on human chromosome I. A comparison of the statistical maps of chromosome I and of the X-chromosome with the cytogenetic maps of these chromosomes at metaphase indicates th...
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ژورنال
عنوان ژورنال: Pediatric Research
سال: 1981
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-198104001-00781